The Heat Start Multi-Purpose LAMP/RT-LAMP 2X Master Mix is designed to provide a simple, one-step solution for LAMP/RT LAMP of DNA or RNA targets. LAMP and RT-LAMP are commonly used isothermal amplification techniques that provide rapid detection of a target nucleic acid using LAMP-specific primers (supplied by the user) and a strand-displacing DNA polymerase. This master mix features a blend of Bst Heat Start DNA Polymerase and Thermo Start Reverse Transcriptase in an optimized LAMP buffer solution.
Both Bst Heat Start DNA Polymerase and Thermo Start Reverse Transcriptase have been engineered for improved performance in LAMP and RT-LAMP reactions. Heat Start Multi-Purpose LAMP/RT-LAMP 2X Master Mix is fully buffered and compatible with different sample types, enabling multiple detection methods. Including turbidity detection, real-time fluorescence detection (when used with LAMP fluorescent dye) and end-point visualization such as colorimetric detection via a metal indicator (e.g., hydroxynaphthol blue.)