One-Step RT-PCR Mix is designed for performing highly sensitive and specific RT-PCR. The kit is based on a genetically engineered reverse transcriptase with enhanced thermal stability resulting in an increased specificity, high cDNA yield and an improved efficiency for highly structured and long cDNA fragments.
The kits contain all reagents required for RT-PCR (except template and primer) in one kit combining simple handling with high flexibility. The premium quality polymerases, ultrapure dNTPs and the optimized complete reaction buffers ensure superior amplification results.
RT-PCR is used to amplify double-stranded DNA from single stranded RNA templates. In the RT step the reverse transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. In the first cycle of the PCR step synthesis, Taq DNA polymerase synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template. During subsequent rounds of cycling the DNA polymerase exponentially amplifies the double-stranded DNA template.
In one-step RT-PCR all components of reverse transcription (RT) and PCR are mixed in one tube prior to starting the reaction and thus carried out sequentially without opening the tube. This offers tremendous convenience when applied to analysis of single targets from multiple samples of RNA and minimizes the risk of contaminations.