APS SYBR Green/ROX qPCR Master Mix (2X) is a ready to use
solution optimized for quantitative real time PCR and two-step
real time RT-PCR on most real time PCR machines. The master
mix includes hot start Taq DNA polymerase, SYBR Green dye I
and dNTPs in an optimized PCR buffer and is supplemented
with ROX passive reference dye. Only template and primers
need to be added.
Hot start Taq DNA polymerase in combination with the
optimized buffer ensures PCR specificity and sensitivity. The
SYBR Green I intercalating dye allows for DNA detection and
analysis without using sequence specific probes. dUTP is
included in the mix for optional carryover contamination
control using uracil-DNA glycosylase (UDG).
The use of SYBR Green/ROX qPCR Master Mix (2X) in real time
PCR ensures reproducible, sensitive and specific quantification
of genomic, plasmid, viral and cDNA templates.