APS SYBR Green/ROX qPCR Master Mix (2X) is a ready to use solution optimized for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes hot start Taq DNA polymerase, SYBR Green dye I and dNTPs in an optimized PCR buffer and is supplemented with ROX passive reference dye. Only template and primers need to be added.
Hot start Taq DNA polymerase in combination with the optimized buffer ensures PCR specificity and sensitivity. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG).
The use of SYBR Green/ROX qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.