DGD is a complete, nontoxic and ready-to-use reagent for the isolation of genomic DNA from various biological sources of animal, plant, yeast, and bacterial origin. The DGD Reagent procedure can be completed in typically 10-30 minutes with DNA recoveries of 70-100%.
In the DGD protocol, a biological sample is homogenized (or lysed) in DGD, and the DNA is precipitated with ethanol, washed and dissolved in 8 mM NaOH. Following pH adjustment, the DNA can be used immediately for analysis or stored at 4°C. The entire isolation can be completed in 20-30 min, and a wide range of DNA molecules can be isolated including genomic DNA and DNA fragments down to 0.1 kb in length. If necessary, samples can be stored in DGD at room temperature for extended periods of time. The isolated DNA is ready for PCR, Southern blotting and other molecular biology applications. Without any additional purification.