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ELISA Blocking Buffer (5% BSA in PBST)

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Buffered BSA solution used to block ELISA strips and as a diluent of primary and secondary antibodies used for ELISA applications. BSA is useful for saturating excess protein-binding sites on membranes and micro plates in immunoassays. Blocker BSA is usually more effective than non-fat milk blocking buffers for biotin avidin systems. Use as a diluent for antigens, antibodies, and other proteins only after sensitization of the micro well plate or membrane.

Description

Buffered BSA solution used to block ELISA strips and as a diluent of primary and secondary antibodies used for ELISA applications. BSA is useful for saturating excess protein-binding sites on membranes and micro plates in immunoassays. Blocker BSA is usually more effective than non-fat milk blocking buffers for biotin avidin systems. Use as a diluent for antigens, antibodies, and other proteins only after sensitization of the micro well plate or membrane.