Take 500µL of H.O.S solution in a small test tube. Add 100µL
of liquefied semen sample to the H.O.S solutions and mix well.
Keep at 37 degree centigrade for 30 minutes. Take just one
drop on the plain glass slide put the cover slip and exam in
under microscope in 40X objective, calculate percentage of
coiled tail. Normal percentage>60%.