Description
RNA later is an aqueous, non-toxic tissue storage reagent that rapidly permeates tissue to stabilize and protect cellular RNA in situ in unfrozen specimens. Tissue pieces are harvested and immediately submerged in RNA later for storage without jeopardizing the quality or quantity of RNA.
Trim the tissue to be less than 0.5 cm in at least one dimension and simply submerge it in 5 volumes of RNA later solution (e.g., a 0.5 g sample requires about 2.5 mL of RNA later solution). Small organs such as rat kidney, liver, and spleen can be stored whole in RNA later solution