Uracil-DNA Glycosylase (UNG), Thermo labile is a recombinant protein in E. coli, from Artic Cod. The enzyme hydrolyzes uracil glycosidic bonds at U-DNA in single- and double-stranded DNA excising uracil and creating alkali sensitive abasic sites in the DNA. The enzyme is inactive on RNA and native, uracil-free DNA. Since Uracil-DNA Glycosylase (UNG), thermo labile has no metal ion requirements, it is fully active in the presence of EDTA.
Uracil-DNA Glycosylase (UNG), thermo labile can be used with dUTP to eliminate PCR or “LAMP carry over” contaminations from previous DNA synthesis reactions. To make PCR or LAMP products suspect able to degradation, dTTP has to be substituted by dUTP in the PCR/LAMP reaction mix. Subsequent PCR/LAMP reaction mixes must be pre-treated with Uracil-DNA Glycosylase (UNG), thermo labile prior to PCR/LAMP to degrade uracil containing DNA. Native DNA does not contain uracil so that the sample is not degraded by this procedure.